1. Basic Product Profile

• Full IUPAC Enzyme Name: Alkanal monooxygenase (FMN-linked)
• Aliases: Bacterial Luciferase, Vibrio luciferase, EC 1.14.14.3, FMN-dependent alkanal monooxygenase
• CAS Number: 9014-00-0
• EINECS: 232-751-7
• Molecular Formula: Complex protein heterodimer (α subunit C350H550N90O120S10; β subunit C320H510N85O110S8)
• Molecular Weight: Holoenzyme ~77 kDa (α ~40 kDa, β ~37 kDa)
• Chemical Classification: FMN-dependent bioluminescent monooxygenase enzyme, bioluminescence reporter reagent, biochemical detection raw material
• Core Advantages
  Natural luminescent enzyme isolated from luminous marine bacteria (Vibrio fischeri, Vibrio harveyi). Catalyzes oxidation of long-chain fatty aldehydes with reduced FMN and molecular oxygen to emit blue-green bioluminescence at 490 nm. High signal linearity, low background interference, stable lyophilized powder form. Widely applied in microbial rapid detection, reporter gene assays, ATP quantification, drug screening and biosensor construction.

2. Physical & Chemical Properties

2.1 Physical Index

1. Appearance: White to pale yellow lyophilized crystalline powder
2. Odor: Odorless
3. Solubility: Freely soluble in neutral aqueous buffer; insoluble in acetone, ethanol, organic solvents
4. Optimum Reaction Temperature: 20–28 °C
5. Optimum pH: 6.8–7.8
6. Storage Temperature: Long-term -20 °C; short-term 2–8 °C
7. Hygroscopy: Strongly hygroscopic, must be sealed against moisture
8. Emission Wavelength: 490 nm blue-green light

2.2 Chemical Properties

1. Heterodimer structure: Composed of α catalytic subunit and β auxiliary subunit, FMN as coenzyme prosthetic group
2. Catalytic reaction: FMNH2 + RCHO + O2 → FMN + RCOOH + H2O + light (490 nm)
3. Thermal lability: Irreversibly denatured above 40 °C; high temperature completely loses luminescent activity
4. pH sensitivity: Rapid activity loss below pH 6.0 or above pH 8.5
5. Photostability: Weak light exposure stable; long intense UV irradiation reduces enzyme activity
6. Inhibitors: Heavy metal ions, strong oxidants, high-concentration denaturants (urea, SDS)
7. Compatibility: Compatible with neutral phosphate, HEPES biological buffers; incompatible with strong acid, strong alkali, organic denaturants

3. Main Application Fields

3.1 Biochemical Luminescence Detection (Core Usage)

1. Rapid microbial total count detection for food, water and pharmaceutical cleanroom monitoring;
2. ATP bioluminescence assay for cell viability, cytotoxicity high-throughput drug screening.

3.2 Molecular Biology Reporter System

3.3 Biosensor & Environmental Monitoring

3.4 Diagnostic Kit Raw Material

4. Quality Specification Table