1. Basic Product Profile

• Full IUPAC Name: tetrasodium 1,3,6,8-pyrenetetrasulfonate
• Aliases: PTS, Pyrene tetrasulfonic acid tetrasodium salt, HPTS, Pyranine
• CAS Number: 59572-10-0
• EINECS: N/A
• Molecular Formula: C16H4Na4O12S4
• Molecular Weight: 610.41
• Chemical Classification: Water-soluble polycyclic aromatic fluorescent dye, pH fluorescent probe, biological staining reagent, industrial fluorescent tracer
• Core Advantages
  Highly water-soluble anionic pyrene derivative with four sulfonate groups, excellent fluorescence quantum yield. Strong pH responsiveness in aqueous media; stable in wide pH range, low cell toxicity, no permeation through cell membranes. Widely used as ratiometric pH sensor for intracellular/extracellular fluid testing, fluorescent tracer for water flow detection, fluorescent stain for polymer and biological research, and standard fluorescent reference material for analytical laboratories.

2. Physical & Chemical Properties

2.1 Physical Index

1. Appearance: Bright yellow to orange crystalline powder
2. Odor: Odorless
3. Solubility: Freely soluble in water; slightly soluble in methanol; insoluble in ethanol, acetone, ether, aliphatic and aromatic non-polar solvents
4. Melting characteristic: Decomposes above 300 °C without distinct melting point
5. Fluorescence spectrum: Excitation λmax = 405 nm; dual emission peaks 430 nm (acid form) / 510 nm (base form)
6. pH response range: pH 5–9 linear ratiometric fluorescence change
7. Hygroscopy: Moderately hygroscopic under high humidity air
8. Aqueous solution color: Yellow-green fluorescent solution under UV light

2.2 Chemical Properties

1. Tetrasulfonate anionic structure: Four sodium sulfonate groups grafted on pyrene core, endowing complete water solubility
2. pH-sensitive protonation: Central pyrene hydroxyl group undergoes reversible protonation, shifts fluorescence emission wavelength for ratiometric detection
3. Photostability: Good light fastness compared to common fluorescent dyes; prolonged strong UV irradiation causes slight fluorescence attenuation
4. Acid & Alkali Stability: Stable across pH 2–12; sulfonate groups do not hydrolyze under weak acid/alkali heating
5. Thermal Stability: No decomposition below 200 °C; high temperature over 280 °C breaks pyrene conjugated skeleton
6. Compatibility: Compatible with aqueous buffer solutions, anionic surfactants; incompatible with cationic polymers, cationic dyes, heavy metal ions (causes precipitation)

3. Main Application Fields

3.1 Biochemical & Cell Research (Core Usage)

1. Ratiometric fluorescent pH probe for cytoplasm, lysosome and extracellular fluid pH measurement;
2. Water-soluble fluorescent marker for liposome, microfluidic and membrane permeability experiments;
3. Fluorescent tracer in cell culture medium with low cytotoxicity.

3.2 Environmental & Hydraulic Testing

3.3 Analytical Chemistry Standard

3.4 Polymer & Coating Auxiliary

4. Quality Specification Table